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KS3 Biology · Photosynthesis & respiration

Leaves

  • The layers of a leaf and the job each one does
  • How a leaf is adapted for photosynthesis
  • Stomata and guard cells: when they open and close
  • Why plants do not breathe: gases move by diffusion
  • 2 min read
  • 5 quick questions
  • Free homework sheet
  • Years 7 to 9
lightWaxy cuticleUpper epidermisPalisade layerSpongy layerAir spaceVein (xylem and phloem)Lower epidermisGuard cellStoma

Revision notes

Leaves are where most photosynthesis happens. Every layer has a job.

lightWaxy cuticleUpper epidermisPalisade layerSpongy layerAir spaceVein (xylem and phloem)Lower epidermisGuard cellStoma
PartJob
Waxy cuticleA waxy, waterproof layer that cuts down water loss
Upper epidermisClear cells that protect the leaf and let light through
Palisade layerTall cells packed with chloroplasts, for photosynthesis
Spongy layerLoosely packed cells with air spaces between them
Air spaceA gap between cells where gases move round the leaf
VeinXylem brings water in and phloem carries sugars away
Lower epidermisThe bottom layer of cells, with most of the stomata in it
Guard cellChanges shape to open and close the stoma
StomaA tiny hole that lets gases in and out of the leaf

Adapted for photosynthesis

  • Broad and flat: a large surface area absorbs more light.
  • Thin: carbon dioxide only diffuses a short distance.
  • Lots of chloroplasts in the palisade layer, near the top where the light is.
  • Veins: xylem brings water; phloem carries sugars away.
  • Air spaces in the spongy layer let gases reach every cell.
  • Stomata, mostly underneath, let carbon dioxide in and oxygen out.
  • A waxy cuticle and a see-through upper epidermis: less water lost, more light let in.
Stomata and guard cells

Each stoma is opened and closed by two guard cells. Open in the light: carbon dioxide diffuses in; oxygen and water vapour diffuse out. Closed at night, and in hot, dry, windy weather, to save water. The catch: closed stomata let no carbon dioxide in, so photosynthesis slows.

Plants do not breathe

Gases move in and out of the leaf by diffusion, from where there is more of a gas to where there is less. Paint clear nail varnish on a leaf, peel it off and look under a microscope: you can see the guard cells and stomata.

Electron microscope image of a leaf surface, with mouth-shaped stomata among the cells and hairs
A leaf's surface under an electron microscope. The small mouth-shaped openings are stomata. Photo: Louisa Howard (Dartmouth electron microscope facility), Public domain, via Wikimedia Commons

Written to the KS3 national curriculum. Last changed 5 November 2026.

Key words

Say them, spell them, use them in an answer.

  • waxy cuticle
  • guard cells
  • diffusion

Common misconception

Often said“Plants breathe in carbon dioxide.”

In factPlants do not breathe. Gases diffuse in and out of the leaf through the stomata.

Giant water lily leaves floating on a pond, each one a big green disc with a turned-up rim

Did you know?

Giant water lily

Its floating leaves can be up to 3 metres across. The underside sits in the water, so its stomata are on the top surface, facing the air.

Photo: 663highland, CC BY 2.5, via Wikimedia Commons

Try 5 questions

Quick questions on leaves, marked as you go. Each one explains the answer.

Question 1 of 5

Why is the upper epidermis see-through?

Choose an answer. Each question explains itself.

The homework sheet

About twenty minutes. Everything on it is in the notes above; the answers are on the last page.

The first page of the leaves homework sheet

Free homework sheet

Leaves homework sheet

17 questions, about twenty minutes, with the answers on the last page: multiple choice, true or false, short answers, key words to define and one to explain in full.

KS3 Photosynthesis & respiration

Revised the whole unit? There is a 40-mark Photosynthesis & respiration test paper with a mark scheme.

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More in this unit

Photosynthesis & respiration

The whole unit